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Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
ZNF598 Rabbit pAb (A15546)
- Описание
- Характеристики
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Overview
Product name ZNF598 Rabbit pAb Catalog No. A15546 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Zinc-finger proteins bind nucleic acids and play important roles in various cellular functions, including cell proliferation, differentiation, and apoptosis. This protein and Grb10-interacting GYF protein 2 have been identified as a components of the mammalian 4EHP (m4EHP) complex. The complex is thought to function as a translation repressor in embryonic development.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 120-420 of human ZNF598 (NP_835461.2). Sequence PRCPELPPFSLFGDLEQHMRRQHELFCCRLCLQHLQIFTYERKWYSRKDLARHRMQGDPDDTSHRGHPLCKFCDERYLDNDELLKHLRRDHYFCHFCDSDGAQDYYSDYAYLREHFREKHFLCEEGRCSTEQFTHAFRTEIDLKAHRTACHSRSRAEARQNRHIDLQFSYAPRHSRRNEGVVGGEDYEEVDRYSRQGRVARAGTRGAQQSRRGSWRYKREEEDREVAAAVRASVAAQQQEEARRSEDQEEGGRPKKEEAAARGPEDPRGPRRSPRTQGEGPGPKETSTNGPVSQEAFSVTG Gene ID Swiss Prot Synonyms HEL2 Calculated MW 99kDa Observed MW 115kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:200 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples A-549, A-431, Jurkat, HeLa, LO2 Cellular location cytosol 
Western blot analysis of extracts of various cell lines, using ZNF598 antibody (A15546) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15S. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 120-420 of human ZNF598 (NP_835461.2). Isotype IgG