быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Zfp541 Rabbit pAb (A15530)
- Описание
- Характеристики
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Overview
Product name Zfp541 Rabbit pAb Catalog No. A15530 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Predicted to enable transcription corepressor activity. Predicted to be involved in histone deacetylation; negative regulation of transcription, DNA-templated; and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within cell differentiation and spermatogenesis. Located in nucleus. Part of histone deacetylase complex. Orthologous to human ZNF541 (zinc finger protein 541).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 108-269 of mouse Zfp541 (NP_001092747.1). Sequence ALKVPKEADEGGRATGSTRKGKRQHSSPQNPLLDCSLCGKVFSSASSLSKHYLTHSQERKHVCKVCSKAFKRQDHLTGHMLTHQKTKPFVCIEQGCSKSYCDYRSLRRHYEVQHGVCILKETPPEEEAYGDPTHNHDVANQPPPSGLRSLGPPEARSPGSVL Gene ID Swiss Prot Synonyms Znf541; EG666528 Calculated MW 141kDa/148kDa Observed MW 170kDa Applications
Reactivity Mouse, Rat Tested applications WB Recommended dilution - WB 1:200 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples mouse brain, mouse testis, rat brain, rat testis Cellular location Nucleus 
Western blot analysis of extracts of various cell lines, using ZNF541 antibody (A15530) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 108-269 of mouse Zfp541 (NP_001092747.1). Isotype IgG