быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
SARS-CoV-2 N Protein Rabbit mAb, BSA and glycerol free (A20021)
- Описание
- Характеристики
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Overview
Product name SARS-CoV-2 N Protein Rabbit mAb, BSA and glycerol free Catalog No. A20021 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC5077-02 Background
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host SMAD3.Immunogen information
Immunogen Recombinant fusion protein of SARS-CoV-2 Nucleoprotein Full length（P59595）. Sequence MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA Gene ID Swiss Prot Synonyms Nucleoprotein; NP Calculated MW 46kDa Observed MW Applications
Reactivity SARS-CoV-2 Tested applications WB IF/ICC DB Recommended dilution - DB 1:500 - 1:2000
- WB 1:1000 - 1:5000
- IF/ICC 1:50 - 1:200
Storage buffer Store at 4℃ Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, pH7.3.Key application Western blotting Immunofluorescence Positive samples Cellular location Customer validation WB(SARS-CoV-2, Mus musculus, Homo sapiens, Staphylococcus aureus)
IHC(Homo sapiens, SARS-CoV-2, Rattus norvegicus)
IF( Homo sapiens)
IF(Homo sapiens)
Western blot analysis of recombinant SARS-COV-2 N Protein, using SARS-CoV-2 N Protein Rabbit mAb, BSA and glycerol free (A20021) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 ng/10 ng/5 ng/2 ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity SARS-CoV-2 Immunogen Recombinant fusion protein of SARS-CoV-2 Nucleoprotein Full length（P59595）. Isotype IgG