![[KD Validated] TOLLIP Rabbit mAb (A23780)](/upload/resize_cache/iblock/3bf/340_340_140cd750bba9870f18aada2478b24840a/roodt0vx6btrqa8o0g1c6avkirlz0nqh.jpg "[KD Validated] TOLLIP Rabbit mAb (A23780)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] TOLLIP Rabbit mAb (A23780)
- Описание
- Характеристики
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Overview
Product name [KD Validated] TOLLIP Rabbit mAb Catalog No. A23780 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC61374 Background
This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. Several transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant protein of human TOLLIP. Sequence Email for sequence Gene ID Swiss Prot Synonyms TOLLIP; IL-1RAcPIP; toll-interacting protein Calculated MW 22kDa/30kDa Observed MW 33kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples 293T, SW260, Mouse testis, Rat testis Cellular location Cytoplasm 
Western blot analysis of various lysates, using [KD Validated] TOLLIP Rabbit mAb (A23780) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of extracts from wild type(WT) and TOLLIP knockdown (KD) 293T cells, using [KD Validated] TOLLIP Rabbit mAb (A23780) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded mouse testis using [KD Validated] TOLLIP Rabbit mAb (A23780) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat testis using [KD Validated] TOLLIP Rabbit mAb (A23780) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human TOLLIP. Isotype IgG