![[KD Validated] PHF11 Rabbit mAb (A23994)](/upload/resize_cache/iblock/d9a/340_340_140cd750bba9870f18aada2478b24840a/xbijv4s9dld9nse34oll8ev8kgzgja22.jpg "[KD Validated] PHF11 Rabbit mAb (A23994)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] PHF11 Rabbit mAb (A23994)
- Описание
- Характеристики
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Overview
Product name [KD Validated] PHF11 Rabbit mAb Catalog No. A23994 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC62550 Background
This gene encodes a protein containing a PHD (plant homeodomain) type zinc finger. This gene has been identified in some studies as a candidate gene for asthma. Naturally-occurring readthrough transcription may occur from the upstream SETDB2 (SET domain bifurcated 2) gene to this locus. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 40-321 of human PHF11（NP_001035533.1）. Sequence MEKRTCALCPKDVEYNVLYFAQSENIAAHENCLLYSSGLVECEDQDPLNPDRSFDVESVKKEIQRGRKLKCKFCHKRGATVGCDLKNCNKNYHFFCAKKDDAVPQSDGVRGIYKLLCQQHAQFPIIAQSAKFSGVKRKRGRKKPLSGNHVQPPETMKCNTFIRQVKEEHGRHTDATVKVPFLKKCKEAGLLNYLLEEILDKVHSIPEKLMDETTSESDYEEIGSALFDCRLFEDTFVNFQAAIEKKIHASQQRWQQLKEEIELLQDLKQTLCSFQENRDLMS Gene ID Swiss Prot Synonyms APY; BCAP; IGEL; IGER; IGHER; NYREN34; NY-REN-34 Calculated MW 38kDa Observed MW 34kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:1000 - 1:5000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, Mouse spleen Cellular location Nucleus 
Western blot analysis of Mouse spleen, using PHF11 Rabbit mAb (A23994) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from wild type(WT) and PHF11 knockdown (KD) 293T cells, using PHF11 Rabbit mAb (A23994) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 40-321 of human PHF11（NP_001035533.1）. Isotype IgG