- 100 μL
- 200 μL
- Описание
- Характеристики
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Overview
Product name ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Catalog No. AS076 Host species Goat Purification method Affinity purification Isotype IgG Background
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.Immunogen information
Immunogen Mouse IgG Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW Applications
Reactivity Tested applications IF/ICC FC Recommended dilution - IF/ICC 1:50 - 1:200
- FC 1:100 - 1:800
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.Key application Immunofluorescence Flow Cytometry Positive samples Cellular location Customer validation IF(Mus musculus, Homo sapiens, Crassostrea gigas, Rattus norvegicus, Ostreidae)
ICC(Crassostrea gigas)
IHC(Crassostrea gigas)
FC(Crassostrea gigas)

Confocal imaging of HeLa cells using TriMethyl-Histone H3-K9 Rabbit mAb (A22295, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:100)(Red), ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L)(AS076, dilution 1:200) (Green)

Flow cytometric analysis of Positive antibody (AE005) (2.5μg/mL) in various cells (orange) compare to Rabbit isotype control (blue) and non-staining control (Red).The secondary antibody used was ABflo® 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (AS076) at 1:100.
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Key applications Immunofluorescence, Flow Cytometry Host species Коза Antibody type Secondary Antibodies Immunogen Mouse IgG Isotype IgG







