быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
HRP Goat Anti-Human IgG (H+L) (AS002)
- Описание
- Характеристики
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Overview
Product name HRP Goat Anti-Human IgG (H+L) Catalog No. AS002 Host species Goat Purification method Affinity purification Isotype Horseradish peroxidase conjugated IgG Background
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.Immunogen information
Immunogen Human IgG Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW 26kDa/55kDa Applications
Reactivity Tested applications WB IHC-P Recommended dilution - WB 1:5000 - 1:100000
- IHC-P 1:500 - 1:5000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples Human IgG Cellular location Customer validation ELISA(Homo sapiens)
IHC(Homo sapiens)
WB(Mesocricetus auratus, Rattus norvegicus, Anatinae, Homo sapiens)

Western blot analysis of extracts of Human IgG, using HRP Goat Anti-Human IgG (H+L) antibody (AS002) at 1:2000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting, Immunohistochemistry Host species Коза Antibody type Secondary Antibodies Immunogen Human IgG Isotype IgG







