- 100 μL
- Описание
- Характеристики
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Overview
Product name ABflo® 555-conjugated Goat Anti-Mouse IgG (H+L) Catalog No. AS057 Host species Goat Purification method Affinity purification Isotype IgG Background
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.Immunogen information
Immunogen Mouse IgG Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW Applications
Reactivity Tested applications IF/ICC FC Recommended dilution - IF/ICC 1:100 - 1:500
- FC 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Immunofluorescence Flow Cytometry Positive samples Cellular location Customer validation IF(Homo sapiens, Danio rerio , Rattus norvegicus)
WB(Mus musculus,Homo sapiens)

Immunofluorescence analysis of HeLa cells using ABflo® 555-conjugated Goat Anti-Mouse IgG (H+L) (AS057) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ABflo® 555-conjugated Goat Anti-Mouse IgG (H+L) (AS057) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using ABflo® 555-conjugated Goat Anti-Mouse IgG (H+L) (AS057) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining.

Flow cytometric analysis of Positive antibody Human Calcitonin R (2.5μg/mL) in various cells (orange) compare to Mouse isotype control (blue) and non-staining control (Red). The secondary antibody used was ABflo® 555-conjugated Goat Anti-Mouse IgG (H+L) (AS057) at 1:100.
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Key applications Immunofluorescence, Flow Cytometry Host species Коза Antibody type Secondary Antibodies Immunogen Mouse IgG Isotype IgG







