быстрая доставка ~4 недели
Размер
- 100 μL
ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) (AS074)
- Описание
- Характеристики
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Overview
Product name ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) Catalog No. AS074 Host species Goat Purification method Affinity purification Isotype ABflo™ 594 conjugated IgG Background
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.Immunogen information
Immunogen Rabbit IgG Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW Applications
Reactivity Tested applications IF/ICC FC Recommended dilution - FC 1:100 - 1:800
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Immunofluorescence Positive samples Cellular location Customer validation IF(Homo sapiens, Ctenopharyngodon idellus, Mus musculus, Ctenopharyngodon idella)
IHC(Mus musculus)
Immunofluorescence analysis of NIH/3T3 using MonoMethyl-Histone H3-K9 Rabbit mAb (A22079) (40x lens), the secondary antibody was ABflo® 594-conjugated AffiniPure Goat Anti antibody (AS074) used at dilution of 1 : 200. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 using MonoMethyl-Histone H3-K9 Rabbit mAb (A22079) (40x lens) , the secondary antibody was ABflo® 594-conjugated AffiniPure Goat Anti antibody (AS074) used at dilution of 1 : 200. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa using MonoMethyl-Histone H3-K9 Rabbit mAb (A22079) (40x lens) , the secondary antibody was ABflo® 594-conjugated AffiniPure Goat Anti antibody (AS074) at dilution of 1 : 200. Blue: DAPI for nuclear staining.
Flow cytometric analysis of Positive antibody E-Cadherin Rabbit mAb (2.5μg/mL) in various cells (orange) compare to Rabbit rabbit isotype control (blue) and non-staining control (Red).The secondary antibody used was ABflo® 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (AS074) at 1:100.
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Key applications Flow Cytometry, Immunofluorescence Host species Коза Antibody type Secondary Antibodies Immunogen Rabbit IgG Isotype IgG