быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
Goat Anti-Human IgG (H+L) (AS084)
- Описание
- Характеристики
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Overview
Product name Goat Anti-Human IgG (H+L) Catalog No. AS084 Host species Goat Purification method Affinity purification Isotype IgG Background
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.Immunogen information
Immunogen Human IgG Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW 25kDa/55kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples Human plasma Cellular location 
Western blot analysis of extracts of Human plasma, using Goat Anti-Human IgG (H+L) antibody (AS084 ) at 1:1000 dilution.
Secondary antibody: HRP Donkey Anti-Goat IgG (H+L) (AS031) at 1:4000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting Host species Коза Antibody type Secondary Antibodies Reactivity Человек Immunogen Human IgG Isotype IgG







