быстрая доставка ~4 недели
Размер
- 100 μL
HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain (AS063)
- Описание
- Характеристики
-
Overview
Product name HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain Catalog No. AS063 Host species Goat Purification method Affinity purification Isotype IgG Background
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.Immunogen information
Immunogen Rabbit IgG Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW 55kDa Applications
Reactivity Tested applications WB Recommended dilution - WB 1:1000-10000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 50% glycerol, pH7.3.Key application Western blotting Positive samples Rabbit IgG, Mouse IgG Cellular location Customer validation WB(Sus scrofa, Mus musculus, Homo sapiens, Rattus norvegicus, Xenopus laevis, Triticum aestivum, Oryctolagus cuniculus)
IHC(Mus musculus)
co-IP(Xenopus laevis)
IF(Homo sapiens)

Western blot analysis of extracts of Rabbit and Mouse IgG, using HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain antibody (AS063) at 1:5000 dilution.
Lysates/proteins: 100ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Коза Antibody type Secondary Antibodies Immunogen Rabbit IgG Isotype IgG







