быстрая доставка ~4 недели
Размер
- 100 μL
HRP Goat Anti-Rabbit IgG (H+L) (AS014)
- Описание
- Характеристики
-
Overview
Product name HRP Goat Anti-Rabbit IgG (H+L) Catalog No. AS014 Host species Goat Purification method Affinity purification Isotype Horseradish peroxidase conjugated IgG Background
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.Immunogen information
Immunogen Rabbit IgG Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW Applications
Reactivity Tested applications WB IHC-P ELISA Recommended dilution - ELISA 1:5000 - 1:10000
- WB 1:2000 - 1:10000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples Cellular location Customer validation WB(Drosophila, Homo sapiens, Mus musculus, Rattus norvegicus, Brachyura, Oryza sativa, Crassostrea gigas, Saccharomyces cerevisiae, Caenorhabditis Elegans, Sus scrofa, Gallus gallus, Oyster, Cavia porcellus, Squaliobarbus curriculus, Oryctolagus cuniculus, Arabidopsis thaliana, M. truncatula, Malus micromalus, Tegillarca granosa, Zea mays, Drosophila melanogaster, Populus davidiana×P.bolleana, Riemerella anatipestifer, Spodoptera frugiperda, Giardia duodenalis, Ictalurus punctatus, Triticum aestivum L., Eospalax cansus, Thale cress, Solanum tuberosum, Cryptosporidium parvum, broiler embryos, Ctenopharyngodon idellus, fenugreek, Toxoplasma gondii, Danio rerio, Blood clams, coli strains, Cyprinus carpio, Escherichia coli, Plutella xylostella, Haliotis, Danio rerio , Nicotiana tabacum, Bos Taurus，Homo sapiens，Mus musculus, Chlamys nobilis, Chlorocebus aethiops , insect, Xenopus laevis, Phytophthora capsici, Anatinae , N. benthamiana, acific oyster, Hamster, C. elegans, Nicotiana benthamiana, Mesocricetus auratus, Carassius auratus gibelio, Mus musculus;Homo sapiens, Bombyx mori Linnaeus, Gossypium spp, Mus musculus、Sus scrofa, gga, acyg, Bos taurus, Capra hircus, Chlorocebus sabaeus, Arabidopsis thaliana Columbia, Ostrinia furnacalis , Ovis aries, Sus domesticus, Ctenopharyngodon idella, A. fumigatus, Pseudosciaena crocea, Pyropia yezoensis, Zea mays L. , Chlorocebus aethiops, Actinopterygii, Caprinae, 无, Staphylococcus aureus, Ark clam)
IHC(Mus musculus, Homo sapiens)
IHC(Homo sapiens, Scophthalmus maximus, Mus musculus, Rattus norvegicus, Bos taurus, Capra hircus, Misgurnus anguillicaudatus, Cervus nippon)
ICC(Rattus norvegicus, Homo sapiens)
ICC(Mus musculus)
IP(Mus musculus)
IF(Mus musculus, Homo sapiens, Felis catus, Homo sapiens、Mus musculus, Rattus norvegicus)
IF(Homo sapiens, Rattus norvegicus, Gallus gallus, Capra hircus)
ELISA(Grass carp, Zea mays, Sus scrofa, SARS-CoV-2)
IHC(Rattus norvegicus, Mus musculus)
IF(Homo sapiens, Mus musculus)
ELISA(Rattus norvegicus)
BrdU(Homo sapiens)
Co-IP(Xenopus laevis)
PAMPs binding assa(OystersC. gigas)
WB(Rattus norvegicus, Homo sapiens)
ELISA(Carassius auratus gibelio, Mus musculus)
MTT(Pleuronectiformes)
FC(Homo sapiens)
Elisa(Homo sapiens)
co-ip(Homo sapiens)
WB (Rattus norvegicus,Bos taurus, Capra hircus)
PCR(Homo sapiens)
WB(Homo sapiens, Misgurnus anguillicaudatus)
WB，IHC(chicken)
Western Blotting(Homo sapiens)
Western Blot(Mus musculus)
PE(Mus musculus)
DB(Homo sapiens)
WB，ELISA(Crassostrea gigas)
FC(Homo sapiens)
Western blot analysis of extracts of HeLa cells, using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s. -
Key applications Western blotting, Immunohistochemistry Host species Коза Antibody type Secondary Antibodies Immunogen Rabbit IgG Isotype IgG