быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
ZNF264 Rabbit pAb (A14089)
- Описание
-
Overview
Product name ZNF264 Rabbit pAb Catalog No. A14089 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a zinc finger protein and belongs to the krueppel C2H2-type zinc-finger protein family. Zinc finger proteins are often localized in the nucleus, bind nucleic acids, and regulate transcription.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ZNF264 (NP_003408.1). Sequence MAAAVLTDRAQVSVTFDDVAVTFTKEEWGQLDLAQRTLYQEVMLENCGLLVSLGCPVPKAELICHLEHGQEPWTRKEDLSQDTCPGDKGKPKTTEPTTCEPALSEGISLQGQVTQGNSVDSQLGQAEDQDGLSEMQEGHFRPGIDPQEKSPGKMSPECDGLGTADGVCSRIGQEQVSPGDRVRSHNSCESGKDPMIQEEENNFKCSECGKVFNKKHLLAG Gene ID Swiss Prot Synonyms ZNF264 Calculated MW 70kDa Observed MW 71kDa Applications
Reactivity Human, Mouse, Rat Tested applications WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples Jurkat, 293T, SW480, SW620 Cellular location Nucleus ZNF264 Rabbit pAb images
Western blot - ZNF264 Polyclonal Antibody (A14089)
Western blot analysis of extracts of various cell lines, using ZNF264 antibody (A14089) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry - ZNF264 Polyclonal Antibody (A14089)
Immunohistochemistry of paraffin-embedded human kidney using ZNF264 antibody (A14089) at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNF264 Polyclonal Antibody (A14089)
Immunohistochemistry of paraffin-embedded human gastric using ZNF264 antibody (A14089) at dilution of 1:100 (40x lens).
Immunofluorescence - ZNF264 Polyclonal Antibody (A14089)
Immunofluorescence analysis of NIH-3T3 cells using ZNF264 antibody (A14089) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - ZNF264 Polyclonal Antibody (A14089)
Immunofluorescence analysis of U-2 OS cells using ZNF264 antibody (A14089) at dilution of 1:100. Blue: DAPI for nuclear staining.* For research use only. Not for therapeutic or diagnostic purposes.
Product Protocols