![[KO Validated] AMPKα1 Rabbit pAb (A18855)](/upload/resize_cache/iblock/6ab/340_340_140cd750bba9870f18aada2478b24840a/834ch0w1vsqje3jzqlei0v6jedbdw7yb.jpg "[KO Validated] AMPKα1 Rabbit pAb (A18855)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 50 μL
[KO Validated] AMPKα1 Rabbit pAb (A18855)
- Описание
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Overview
Product name [KO Validated] AMPKα1 Rabbit pAb Catalog No. A18855 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]Immunogen information
Immunogen Recombinant protein of human PRKAA1. Gene ID Swiss Prot Synonyms AMPK; AMPKa1 Calculated MW 64kDa/65kDa Observed MW 64kDa Applications
Reactivity Human, Mouse Tested applications WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP Recommended dilution WB 1:500 - 1:2000 Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location [KO Validated] AMPKα1 Rabbit pAb images
Western blot - [KO Validated] AMPKα1 Rabbit pAb (A18855)
Western blot analysis of extracts from normal (control) and AMPKα1 knockout (KO) HeLa cells, using AMPKα1 antibody (A18855) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.* For research use only. Not for therapeutic or diagnostic purposes.
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