![[KO Validated] 14-3-3 sigma Rabbit pAb (A18011)](/upload/resize_cache/iblock/d6b/340_340_140cd750bba9870f18aada2478b24840a/85b3dagj4jrulhlg34an6e4hqfhkpxkr.jpg "[KO Validated] 14-3-3 sigma Rabbit pAb (A18011)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
[KO Validated] 14-3-3 sigma Rabbit pAb (A18011)
- Описание
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Overview
Product name [KO Validated] 14-3-3 sigma Rabbit pAb Catalog No. A18011 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human 14-3-3 sigma (NP_006133.1). Sequence MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVREYREKVETELQGVCDTVLGLLDSHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQEPQS Gene ID Swiss Prot Synonyms SFN; YWHAS; stratifin Calculated MW 24kDa/27kDa Observed MW 27kDa Applications
Reactivity Human, Mouse, Rat Tested applications WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples HeLa Cellular location Cytoplasm, Nucleus, Secreted [KO Validated] 14-3-3 sigma Rabbit pAb images
Western blot - [KO Validated] 14-3-3 sigma Rabbit pAb (A18011)
Western blot analysis of extracts from normal (control) and 14-3-3 sigma knockout (KO) HeLa cells, using 14-3-3 sigma antibody (A18011) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.* For research use only. Not for therapeutic or diagnostic purposes.
Product Protocols