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Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
ZNF217 Rabbit pAb (A7002)
- Описание
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Overview
Product name ZNF217 Rabbit pAb Catalog No. A7002 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ZNF217 (NP_006517.1). Sequence MQSKVTGNMPTQSLLMYMDGPEVIGSSLGSPMEMEDALSMKGTAVVPFRATQEKNVIQIEGYMPLDCMFCSQTFTHSEDLNKHVLMQHRPTLCEPAVLRVEAEYLSPLDKSQVRTEPPKEKNCKENEFSCEVCGQTFRVAFDVEIHMRTHKDSFTYGCNMCGRRFKEPWFLKNHMRTHNGKSGARSKLQQ Gene ID Swiss Prot Synonyms ZNF217; ZABC1 Calculated MW 115kDa Observed MW 115kDa Applications
Reactivity Human, Mouse, Rat Tested applications WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP Recommended dilution WB 1:500 - 1:2000
IF 1:50 - 1:100Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples DU145, A-549, Mouse thymus, Rat thymus Cellular location Nucleus ZNF217 Rabbit pAb images
Western blot - ZNF217 Polyclonal Antibody (A7002)
Western blot analysis of extracts of various cell lines, using ZNF217 Antibody (A7002) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunofluorescence - ZNF217 Polyclonal Antibody (A7002)
Immunofluorescence analysis of U2OS cells using ZNF217 antibody (A7002).* For research use only. Not for therapeutic or diagnostic purposes.
Product Protocols