- Описание
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Product Information
Description
Mouse monoclonaltoGAPDH
Protein full name
Glyceraldehyde-3-phosphate dehydrogenase
Synonyms
GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase
Immunogen
KLH conjugated synthetic peptide corresponding to Mouse GAPDH
Isotype
IgG1
Purity Affinity purification
Subcellular location Cytoplasm,Nucleus,Membrane Predicted MW.
36 kDa
Observed MW.
36 kDa
Uniprot ID
Applications
Applications
Species
Dilution
Positive Sample
WB
Human, Mouse, Rat
1: 1000-1: 2000
heart, lung, liver, kidney, brain
IHC/IF
Human, Mouse, Rat
1: 500-1: 1000
breast cancer, esophageal cancer, kidney, testis, spinal cord
Background
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.
Images

Western blot analysis of GAPDH (GB15002) at dilution of 1: 2000

Western blot analysis of GAPDH (GB15002)
at dilution of 1: 2000

Immunohistochemistry of paraffin embedded human breast cancer using GAPDH (GB15002) at dilution of 1: 500

Immunohistochemistry of paraffin embedded human esophageal cancer using GAPDH (GB15002) at dilution of 1: 500

Immunohistochemistry of paraffin embedded mouse kidney using GAPDH (GB15002) at dilution of 1: 500

Immunohistochemistry of paraffin embedded rat kidney using GAPDH (GB15002) at dilution of 1: 500

Immunohistochemistry of paraffin embedded mouse testis using GAPDH (GB15002) at dilution of 1: 500

Immunohistochemistry of paraffin embedded rat spinal cord using GAPDH (GB15002) at dilution of 1: 500
Storage
Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).







