- Описание
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Product Information
Description
Rabbit monoclonal to beta Actin antibody
Protein full name
Actin, cytoplasmic 1
Synonyms
ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin
Immunogen
KLH conjugated synthetic peptide corresponding to Mouse beta-Actin
Isotype
IgG1
Purity
Affinity purification
Subcellular location
Cytoplasm
Predicted MW.
42 kDa
Observed MW.
45 kDa
Uniprot ID
P60710
Applications
Applications
Species
Dilution
Positive Sample
WB
Human, Mouse, Rat
1: 1000-1: 2000
HeLa, Jurkat, MCF-7
PC-3, HepG2
IHC/IF
Human, Mouse, Rat
1: 500-1: 1000
spleen, colon cancer, lung, lung cancer
ICC/IF
Human, Mouse, Rat
1: 300-1: 1000
NIH/3T3, A549, etc.
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Images

Western blot analysis of beta-Actin (GB15003) at dilution of 1: 2000 
Immunohistochemistry of paraffin embedded mouse spleen using beta-Actin (GB15003) at dilution of 1: 1000 
Immunohistochemistry of paraffin embedded human colon cancer using beta-Actin (GB15003) at dilution of 1: 1000 
Immunohistochemistry of paraffin embedded human lung cancer using beta-Actin (GB15003) at dilution of 1: 1000 
Immunohistochemistry of paraffin embedded rat lung using beta-Actin (GB15003) at dilution of 1: 1000 Storage
Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).







