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Product Information
| Description | Desmin mouse monoclonal |
| Protein full name | Desmin |
Synonyms | DES, CSM1, CSM2, LGMD2R, desmin |
| Immunogen | Recombinant protein corresponding to Mouse desmin |
| Isotype | IgG |
| Purity | Affinity purification |
| Predicted MW. | 54 kDa |
| Observed MW. | 55kDa |
| Subcellular location | Cytoplasm, Cell membrane |
| Uniprot ID | P31001,P48675 |
Applications
| Applications | WB | IHC, IF | |
| Species | Mouse, Rat | Mouse, Rat | |
| Dilution | 1:1000-1:2000 | 1:200-1:1000 | |
| Positive tissue | Heart, C2C12 cell | Heart, skeletal muscle |
Background
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction.
Images
| Western blot analysis of Desmin (GB12081) at dilution of 1:1000. Lane 1: C2C12 cell lysate Lane 2: Hela cell lysate Lane 3: Raw264.7 cell lysate Lane 4: Mouse heart tissue lysate Lane 5: Mouse spleen tissue lysate Lane 6: Mouse lung tissue lysate Lane 7: Rat heart tissue lysate |
| Immunohistochemistry analysis of parafffin-embedded mouse heart using Desmin (GB12081) at dilution of 1:500 |
| Immunohistochemistry analysis of parafffin-embedded rat skeletal muscle using Desmin (GB12081) at dilution of 1:500 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
