Product Information
Description | XIAP rabbit polyclonal |
Protein full name | E3 ubiquitin-protein ligase XIAP |
Synonyms | Xiap, XIAP, API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3, X-linked inhibitor of apoptosis |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse XIAP |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Nucleus, Cytoplasm |
Uniprot ID |
Applications
Applications | Species | Dilution | Positive Tissue |
IHC | Human | 1: 500-1: 1000 | lung cancer, colon cancer |
IF | Human | 1: 250-1: 500 | lung cancer, colon cancer |
Background
X-linked inhibitor of apoptosis protein (XIAP) is a protein that stops apoptotic cell death. XIAP is a member of the inhibitor of apoptosis family of proteins (IAP). XIAP stops apoptotic cell death that is induced either by viral infection or by overproduction of caspases. Caspases are the enzymes primarily responsible for cell death. XIAP binds to and inhibits caspase 3, 7 and 9. The BIR2 domain of XIAP inhibits caspase 3 and 7, while BIR3 binds to and inhibits caspase 9. The RING domain utilizes E3 ubiquitin ligase activity and enables IAPs to catalyze ubiquination of self, caspase-3, or caspase-7 by degradation via proteasome activity. However, mutations affecting the RING Finger do not significantly affect apoptosis, indicating that the BIR domain is sufficient for the protein’s function. When inhibiting caspase-3 and caspase-7 activity, the BIR2 domain of XIAP binds to the active-site substrate groove, blocking access of the normal protein substrate that would result in apoptosis.
Images
| Immunohistochemistry analysis of paraffin-embedded human lung cancer using XIAP (GB11888) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin-embedded human colon cancer using XIAP (GB11888) at dilution of 1: 800 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
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