Product Information
Description | AKT1 rabbit polyclonal |
Protein full name | v-akt murine thymoma viral oncogene homolog 1 |
Synonyms | AKT1, AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, AKT serine/threonine kinase 1 |
Immunogen | Recombinant protein corresponding to Mouse AKT1 |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. | 56 kDa |
Observed MW. | 60 kDa |
Uniprot ID |
Applications
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 1000-1: 1500 | pneumonia, splenitis, thymus |
Background
RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This enzyme belongs to the AKT subfamily of serine/threonine kinases. It is commonly referred to as PKB, or by both names as "Akt/PKB". The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Images
| Western blot analysis of AKT1 (GB11689) at dilution of 1: 1000 Lane 1: Jurkat cell lysate Lane 2: HeLa cell lysate Lane 3: Raw264.7 cell lysate Lane 4: Mouse pneumonia tissue lysate Lane 5: Mouse splenitis tissue lysate Lane 6: Mouse thymus tissue lysate Lane 7: Rat pneumonia tissue lysate Lane 8: Rat splenitis tissue lysate Lane 9: Rat thymus tissue lysate |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
