Product Information
Description | Rad51 rabbit polyclonal |
Protein full name | DNA repair protein RAD51 homolog 1 |
Synonyms | RAD51, BRCC5, FANCR, HHsRad51, HsT16930, MRMV2, RAD51A, RECA, RAD51 recombinase |
Immunogen | Recombinant protein corresponding to Mouse Rad51 |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. | 37 kDa |
Observed MW. | 37 kDa |
Subcellular location | Nucleus, Cytoskeleton |
Uniprot ID |
Applications
applications | species | dilution | positive tissue |
IHC | Mouse, Rat | 1: 500-1: 1000 | testis, ovary, brain |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | thymus |
IF | Mouse, Rat | 1: 500-1: 1000 | testis, ovary, brain |
Background
RAD51 is a eukaryotic gene, which is highly conserved from yeast to humans. The enzyme encoded by this gene is a member of the RAD51 protein family which assists in repair of DNA double strand breaks. In humans, RAD51 is a 339-amino acid protein that plays a major role in homologous recombination of DNA during double strand break repair. In this process, an ATP dependent DNA strand exchange takes place in which a template strand invades base-paired strands of homologous DNA molecules. RAD51 is involved in the search for homology and strand pairing stages of the process.
Images
| Western blot analysis of Rad51 (GB11572) at dilution of 1: 800 Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: U87 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: Mouse thymus tissue lysate |
| Immunohistochemistry analysis of paraffin-embedded mouse testis using Rad51 (GB11573) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded rat ovary using Rad51 (GB11573) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse brain using Rad51 (GB11573) at dilution of 1:1000 |
| Immunohistochemistry analysis of paraffin-embedded rat brain using Rad51 (GB11573) at dilution of 1:1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse sepsis brain using Rad51 (GB11573) at dilution of 1:1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed fetal mouse brain using Rad51 (GB11573) at dilution of 1:1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat brain using Rad51 (GB11573) at dilution of 1:1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat cerebellum using Rad51 (GB11573) at dilution of 1:2000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol |
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