Product Information
Description | TNF-α rabbit polyclonal |
Protein full name | Tumor necrosis factor alpha |
Synonyms | TNF,DIF,TNF-alpha,TNFA,TNFSF2,Tumour necrosis factor, Tumour necrosis factor alpha, TNF-α, tumor necrosis factor, TNLG1 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse TNF-α |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Cell membrane, Secreted |
Predicted MW. | 26 kDa |
Observed MW. | 26 kDa |
Uniprot ID | P01375, P06804 |
Applications
Applications | Species | Dilution | Positive Tissue |
WB | Human | 1: 200-1: 500 | splenitis |
IHC | Mouse | 1: 200-1: 800 | lung |
IF | Mouse | 1: 200-1: 800 | lung |
Background
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Images
| Western blot analysis of TNF-α (GB11188) at dilution of 1: 200 Lane 1: HepG2+LPS cell lysate Lane 2: Mouse splenitis tissue lysate |
| Immunohistochemistry analysis of paraffin-embedded mouse lung using TNF-α(GB11188)at dilution of 1: 200 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
