Product Information
Description | PKM2 rabbit polyclonal |
Protein full name | Pyruvate kinase PKM |
Synonyms | PKM, CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, pyruvate kinase, muscle |
Immunogen | Recombinant protein corresponding to Mouse PKM2 |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. | 58 kDa |
Observed MW. | 58 kDa |
Uniprot ID |
Applications
applications | species | dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | muscle, brain |
Background
Pyruvate kinase isozymes M1/M2 (PKM1/M2), also known as pyruvate kinase muscle isozyme (PKM), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an enzyme that in humans is encoded by the PKM2 gene.Pyruvate kinase catalyzes the last step within glycolysis, the dephosphorylation of phosphoenolpyruvate to pyruvate, and is responsible for net ATP production within the glycolytic sequence. In contrast to mitochondrial respiration, energy regeneration by pyruvate kinase is independent from oxygen supply and allows survival of the organs under hypoxic conditions often found in solid tumors.
Images
| Western blot analysis of PKM2 (GB11392) at dilution of 1:1000. Lane 1:HepG2 cell lysate Lane 2:A549 cell lysate Lane 3:SH-SY5Y cell lysate Lane 4:Mouse muscle tissue lysate Lane 5:Mouse brain tissue lysate Lane 6:Rat muscle tissue lysate Lane 7:Rat brain tissue lysate |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
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