Product Information
Description | Osteopontin rabbit polyclonal |
Protein full name | Osteopontin |
Synonyms | SPP1, BNSP, BSPI, ETA-1, OPN, secreted phosphoprotein 1, Osteopontin |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Osteopontin |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Secreted |
Uniprot ID |
Applications
applications | species | dilution | Positive tissue |
IHC | Mouse, Rat | 1: 500-1: 1000 | bone |
IF | Rat | 1: 500-1: 1000 | brain, substantia nigra |
Background
Osteopontin (OPN), also known as bone sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by theSPP1 gene (secreted phosphoprotein 1).Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.
Images
| Immunohistochemistry analysis of paraffin-embedded mouse bone using Osteopontin (GB11500) at dilution of 1: 1000. |
| Immunohistochemistry analysis of paraffin-embedded mouse bone using Osteopontin (GB11500) at dilution of 1:1000. |
| Immunohistochemistry analysis of paraffin-embedded rat bone using Osteopontin (GB11500) at dilution of 1: 1000. |
| Immunofluorescent analysis of paraformaldehyde-fixed rat brain substantia nigra using Osteopontin (GB11500) at dilution of 1: 500 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
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