Product Information
Description | TLR4 rabbit polyclonal |
Protein full name | Toll-like receptor 4 |
Synonyms | TLR4, ARMD10, CD284, TLR-4, TOLL, toll like receptor 4 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse TLR4. |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. | 95 KDa |
Observed MW. | 95 KDa |
Subcellular location | Membrane |
Uniprot ID |
Applications
applications | species | dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 1000 | spleen, lung, thymus |
IHC | Mouse, Rat | 1: 1000-1: 2000 | spleen, lymph |
IF | Mouse, Rat | 1: 1000-1: 2000 | spleen, lymph |
Background
A member of the toll-like receptor (TLR) family, which plays a fundamental
role in pathogen recognition and activation of innate immunity. It
cooperates with LY96 (also referred as MD-2) and CD14 to mediate
in signal transduction events induced by lipopolysaccharide (LPS) found
in most gram-negative bacteria. Mutations in this gene have been
associated with differences in LPS responsiveness. TLR4 signaling
responds to signals by forming a complex using an extracellular
leucine-rich repeat domain (LRR) and an intracellular toll/interleukin-1
receptor (TIR) domain.
Images
| Western blot analysis of TLR4 (GB11519) at dilution of 1:1000. Lane 1: Jurkat cell lysate Lane 2: Raw264.7 cell lysate Lane 3: A20 cell lysate Lane 4: Mouse spleen tissue lysate Lane 5: Mouse lung tissue lysate Lane 6: Mouse thymus tissue lysate Lane 7: Rat spleen tissue lysate Lane 8: Rat lung tissue lysate Lane 9: Rat thymus tissue lysate |
| Immunohistochemistry analysis of paraffin-embedded mouse spleen using TLR4 (GB11519) at dilution of 1:2000 |
| Immunohistochemistry analysis of paraffin-embedded rat spleen using TLR4 (GB11519) at dilution of 1:2000 |
| Immunohistochemistry analysis of paraffin-embedded rat lymph using TLR4 (GB11519) at dilution of 1:2000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE: