Product Information
Description | SREBP1 rabbit polyclonal |
Protein full name | Sterol regulatory element-binding protein 1 |
Synonyms | SREBF1, SREBP-1c, SREBP1, bHLHd1, SREBP1a, sterol regulatory element binding transcription factor 1 |
Immunogen | Recombinant protein corresponding to Mouse SREBP1. |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. | 121 KDa |
Observed MW. | 54 KDa |
Uniprot ID |
Applications
applications | species | dilution | Positive tissue |
WB | Human, Mouse | 1: 500-1: 1000 | lung, kidney |
Background
SREBP1, also known as SREBF1, a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family, are transcription factors that bind to a sequence in the promoter of different genes, called sterol regulatory element-1 (SRE1). The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription.
Images
| Western blot analysis of SREBP1 (GB11524) at dilution of 1:1000. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: Mouse lung tissue lysate Lane 5: Mouse kidney tissue lysate |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.