Product Information
Description | E2F1 rabbit polyclonal |
Protein full name | Transcription factor E2F1 |
Synonyms | E2F1, E2F-1, RBAP1, RBBP3, RBP3, E2F transcription factor 1 |
Immunogen | Recombinant protein corresponding to Mouse E2F1 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Nucleus |
Uniprot ID |
Applications
applications | species | dilution | positive tissue |
IHC | Mouse, Rat | 1: 500-1: 1000 | brain, spinal cord |
IF | Mouse, Rat | 1: 1000-1: 2000 | spinal cord, brain |
Background
Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain.
Images
| Immunohistochemistry analysis of paraffin-embedded mouse brain using E2F1 (GB11571) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using E2F1 (GB11571) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse spinal cord using E2F1 (GB11571) at dilution of 1: 2000 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse brain using E2F1 (GB11571) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat brain using E2F1 (GB11571) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
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