Артикул
GB111402
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Anti - ZO1 tight junction protein Rabbit pAb
- Описание
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Product Information
Description
ZO1 tight junction protein rabbit polyclonal antibody
Protein full name
Tight junction protein ZO-1
Synonyms
TJP1, ZO-1, Tight junction protein 1
Immunogen
KLH conjugated Synthetic peptide corresponding to Human ZO-1
Isotype
IgG
Purity
Affinity purification
Subcellular location
Cell membrane, Cell junction
Predicted MW.
195 kDa
Observed MW.
220 kDa
Uniprot ID
Q07157, P39447
Applications
Applications
Species
Dilution
Positive Sample
WB
Human, Mouse
1: 500-1: 1000
HEK293, HT29, HepG2, MCF-7, A431
IHC/IF
Human, Mouse, Rat
1: 500-1: 1000
jejunum, kidney, small intestine
Background
Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium. ZO-1 is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells . It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin . ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation.
Images
Western blot analysis of ZO1 tight junction protein (GB111402) at dilution of 1: 500
Immunofluorescence of paraffin embedded human kidney using ZO-1 (GB111402) at dilution of 1: 500 (400x lens) 
Immunofluorescence of paraffin embedded mouse jejunum using ZO-1 (GB111402) at dilution of 1: 500 (400x lens)
Immunofluorescence of paraffin embedded rat small intestine using ZO-1 (GB111402) at dilution of 1: 500 (350x lens) Storage
Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.