быстрая доставка ~4 недели
Размер
- 1000 U
- 200 U
- Описание
-
Description
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated. E. coli DNA Ligase is active at a range of temperatures (4°C - 37°C).Product Components
Components Cat. No. E.coli DNA Ligase (10, 000 U/ml) RM20505 10X E.coli DNA Ligase Reaction Buffer RM20128 Product Information
Feature Specification Product Source Purified from E. coli strain containing a cloned E. coli DNA Ligase gene. Applications Non-Cloning Ligation Unit Definition One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA (5' DNA termini concentration of 0.12 μM, 300 μg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X E. coli DNA Ligase Reaction Buffer. Storage Conditions 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50 % Glycerol, pH7.4@25°C Storage Temperature -20°C Reaction Conditions 1X E.coli DNA Ligase Reaction Buffer, Incubate at 16°C Reaction Buffer or Mix 1X E. coli DNA Ligase Reaction Buffer:
30 mM Tris-HCl, 4 mM MgCl2, 26 μM NAD, 1 mM DTT, 50 μg/ml BSA, pH 8 @ 25°CHeat Inactivation 65°C for 20 min