быстрая доставка ~4 недели
Размер
- 1000 U
- 500 U
- 5000 U
- Описание
-
Description
5'...GmA/TC...3'
3'...CT/mAG...3'Product Components
Components Cat. No. DpnI (20, 000 U/ml) RM21610 10X Buffer CutS RM20103 Product Information
Feature Specification Applications Restriction Enzyme Digestion Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of pBR322 DNA (dam methylated) in 1 hour at 37°C in a total reaction volume of 50 µl. Storage Conditions 10 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 μg/ml BSA, 50% Glycerol pH 7.4 @ 25°C Storage Temperature -20°C Reaction Conditions 1X Buffer CutS, Incubate at 37°C. Reaction Buffer or Mix 1X Buffer CutS:
50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 μg/ml BSA, pH 7.9 @ 25°CHeat Inactivation 80°C for 20 min Methylation Sensitivity dam methylation Not Sensitive dcm methylation Not Sensitive CpG Methylation Blocked by Overlapping Activity in Abclonal Buffers CutA CutB CutC CutS 100% 100% 75% 100%