быстрая доставка ~4 недели
Размер
- 1000 U
- 5000 U
- Описание
-
Description
5'…A/CGCGT...3'
3'…TGCGC/A...5'Product Components
Components Cat. No. MluI (10, 000 U/mL) RM21623 10X Buffer CutC RM20106 Product Information
Feature Specification Applications Restriction Enzyme Digestion Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µL. Storage Conditions 10 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/mL BSA, 50% Glycerol, pH 7.4 @ 25°C Storage Temperature -20°C Reaction Conditions 1X Buffer CutC. Incubate at 37°C. Reaction Buffer or Mix 1X Buffer CutC:
100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 100 μg/mL BSA, pH 7.9 @ 25°CHeat Inactivation 80°C for 20 min. Methylation Sensitivity dam methylation Not Sensitive dcm methylation Not Sensitive CpG Methylation Blocked Activity in Abclonal Buffers CutA CutB CutC CutS 10% 50% 100% 25%