быстрая доставка ~4 недели
Размер
- 1250 U
- 250 U
- Описание
-
Description
T7 Endonuclease I (T7 Endo I) recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5´ to the mismatch. The protein is the product of T7 gene 3.Product Components
Components Cat. No. T7 Endonuclease I (10, 000 U/mL) RM20525 10X ABuffer B RM20126 Product Information
Feature Specification Product Source An E. coli strain that carries T7 Endonuclease I (T7 Endo I). Applications Recognition of mismatched DNA. Resolve four-way junction or branched DNA. Detection or cleavage of heteroduplex and nicked DNA.Random cleavage of linear DNA for shotgun cloning. Unit Definition One unit is defined as the amount of enzyme required to convert > 90% of 1 μg of supercoiled cruciform pUC(AT)* to > 90% linear form in a total reaction volume of 50 μL in 1 hour at 37°C. Storage Conditions 20 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.15% Triton X-100, pH 7.5 @ 25°C Storage Temperature -20°C Reaction Conditions 1X ABuffer B, Incubate at 37°C.
1X ABuffer B: 10 mM Tris-HCl, 50 mM NaCl, 10 mM MgCl2, 1 mM DTT, pH 7.9 @ 25°C