быстрая доставка ~4 недели
Размер
- 20 μg
- 50 μg
Рекомбинантный Noggin человека (Recombinant Human Noggin/NOG Protein), ABclonal
- Описание
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Overview
Product name Recombinant Human Noggin/NOG Protein Catalog No. RP01228 Description Recombinant Human Noggin/NOG Protein is produced by CHO Cells expression system. The target protein is expressed with sequence (Gln28-Cys232) of human Noggin (Accession #NP_005441.1) fused with a 6×His tag at the C-terminus. Bio-Activity 1.Measured by its binding ability in a functional ELISA.Immobilized Human BMP4 at 0.5 μg/mL (100 μL/well) can bind Noggin with a linear range of 16-314 ng/mL.2.Measured by its binding ability in a functional ELISA.Immobilized Human Noggin at 1 μg/mL (100 μL/well) can bind Noggin Rabbit pAb with a linear range of 1-9 ng/mL.3.Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.26-1.06 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4. Purity > 90% by SDS-PAGE. Endotoxin < 1.0 EU/μg of the protein by LAL method. Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Species Human Product information
Synonym Noggin; NOG; SYM1; SYNS1 Expression Host CHO Cells Tag C-His Swiss-Prot Gene ID Usage guide
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. * For research use only. Not for therapeutic or diagnostic purposes.
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