быстрая доставка ~4 недели
Размер
- 100 μg
- 20 μg
- 200 μg
- 5 μg
- 50 μg
Active Recombinant Human HMGB1 Protein (RP00010)
- Описание
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Overview
Product name Active Recombinant Human HMGB1 Protein Catalog No. RP00010 Description Active Recombinant Human HMGB1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Glu215) of human HMGB1 (Accession #NP_002119.1) fused with a 6×His tag at the C-terminus. Bio-Activity Measured by its binding ability in a functional ELISA. Immobilized Recombinant human HMGB1 at 2 μg/mL (100 μL/well) can bind Recombinant human AGER with a linear range of 15-50 ng/mL. Purity > 97% by SDS-PAGE. Endotoxin < 1.0 EU/μg of the protein by LAL method. Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Species Human Background
High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. Posttranslational modification of HMGB1, including acetylation, phosphorylation, and methylation, affects HMGB1 localization, receptor interactions, and bioactivity. HMGB1 can be localized to the nucleus or cytoplasm and can also be secreted despite its lack of a signal peptide. HMGB1 binds DNA in a non-sequence specific manner and may act as a structural cofactor during gene transcription.Acetylation of HMGB1 results in its cytoplasmic localization and eventual secretion. HMGB1 can be secreted by multiple cell types, and it is also released upon cell necrosis, apoptosis, and pyroptosis.Product information
Synonym HMG-1; HMG1; HMG3; SBP-1 Expression Host HEK293 cells Tag C-His Swiss-Prot Gene ID Usage guide
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. * For research use only. Not for therapeutic or diagnostic purposes.
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