быстрая доставка ~4 недели
Размер
- 10 μg
- 100 μg
- 20 μg
- 200 μg
- 5 μg
- 50 μg
Active Recombinant Human Kallikrein-11/KLK11 Protein (RP00149)
- Описание
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Overview
Product name Active Recombinant Human Kallikrein-11/KLK11 Protein Catalog No. RP00149 Description Active Recombinant Human Kallikrein-11/KLK11 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Asn250) of human KLK11/Kallikrein-11 (Accession #NP_006844.1) fused with a 6×His tag at the C-terminus. Bio-Activity Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5, 5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >141.40 pmol/min/μg. Purity > 92% by SDS-PAGE. Endotoxin < 0.1 EU/μg of the protein by LAL method. Formulation Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation. Species Human Background
kallikrein-related peptidase 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family. It is a subgroup of serine proteases with diverse physiological functions, which is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.The KLK11 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19.Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences, respectively. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas. Recombinant human KLK11, after being activated by thermolysin, is active against a thioester substrate. This activity can be inhibited by AEBSF dichloroisocoumarin, and aprotinin.Product information
Synonym PRSS20; TLSP Expression Host HEK293 cells Tag C-His Swiss-Prot Gene ID Usage guide
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. * For research use only. Not for therapeutic or diagnostic purposes.
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