быстрая доставка ~4 недели
Размер
- 10 μg
- 100 μg
- 20 μg
- 50 μg
Active Recombinant Human Mature TGF-beta 1 Protein (RP01458)
- Описание
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Overview
Product name Active Recombinant Human Mature TGF-beta 1 Protein Catalog No. RP01458 Description Active Recombinant Human Mature TGF-beta 1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu30-Ser390(Cys33Ser)) of human Mature TGF-beta 1 (Accession #NP_000651.3) fused with a 8×His tag at the N-terminus. Bio-Activity 1.Measured by its binding ability in a functional ELISA. Immobilized Human TGF-beta 1 at 2 μg/mL (100 μL/well) can bind Human TGFBR2 with a linear range of 0.78-11 ng/mL.2.Recombinant Human Mature TGF-beta 1 induces MCF7 cells EMT with a concentration of 12.5 ng/mL. After 10 h, the cell morphology significantly altered, appearing a fusiform shape (figure 1). After 48 h, the change of EMT associated genes are as figure 2. Results showed that the induction was successful. Purity > 95% by SDS-PAGE. Endotoxin Please contact us for more information. Formulation Lyophilized from a 0.22 μm filtered solution of 50mM Glycine, 150mM Nacl, pH3.5. Species Human Background
TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-beta 1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta 1 also has the opposite effect on cells of myeloid origin. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.Product information
Synonym CED; DPD1; LAP; TGF-beta 1; TGFB; TGFbeta Expression Host HEK293 cells Tag N-his Swiss-Prot Gene ID Usage guide
Storage Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. * For research use only. Not for therapeutic or diagnostic purposes.
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