быстрая доставка ~4 недели
Размер
- 10 μg
- 100 μg
- 20 μg
- 200 μg
- 50 μg
Active Recombinant Human CSF1R/M-CSF R/CD115 Protein (RP00268)
- Описание
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Overview
Product name Active Recombinant Human CSF1R/M-CSF R/CD115 Protein Catalog No. RP00268 Description Active Recombinant Human CSF1R/M-CSF R/CD115 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile20-Glu512) of human CSF1R (Accession #NP_005202.2) fused with a 6×His tag at the C-terminus. Bio-Activity 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human M-CSF at 2 μg/mL (100 μL/well) can bind Recombinant Human CSF1R with a linear range of 50-200 ng/mL. 2.Measured by its ability to inhibit the GCSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this ettect is typically 0.095-0.38 ng/mL. Purity > 95% by SDS-PAGE. Endotoxin < 0.1 EU/μg of the protein by LAL method. Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Species Human Background
The recombinant human CSF1R/GST chimera consists of 667 amino acids with a molecular weight of 76 KDa. It migrates as an about 75 KDa band in SDS-PAGE under reducing conditions.M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages.This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. CSF1R is a receptor for a cytokine called colony stimulating factor 1, The protein encoded by the CSFR1 gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most, if not all, of the biological effects of this cytokine. Ligand binding activates CSFR1 through a process of oligomerization and transphosphorylation . Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of CSF1R1 are found in microglia in Alzheimer's disease and after brain injuries. The role of CSF1 and CSF1R in normal and neoplastic mammary development that may elucidate potential relationships of growth factor-induced biological changes in the breast during pregnancy and tumor progression.Product information
Synonym C-FMS ; CD115 ; CSF-1R ; CSFR ; FIM2 ; FMS ; HDLS ; M-CSF-R ; MCSF Receptor Expression Host HEK293 cells Tag C-His Swiss-Prot Gene ID Usage guide
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. * For research use only. Not for therapeutic or diagnostic purposes.
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