быстрая доставка ~4 недели
Размер
- 10 μg
- 100 μg
- 20 μg
- 200 μg
- 5 μg
- 50 μg
Active Recombinant Human Aminopeptidase N/CD13 Protein (RP00188)
- Описание
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Overview
Product name Active Recombinant Human Aminopeptidase N/CD13 Protein Catalog No. RP00188 Description Active Recombinant Human Aminopeptidase N/CD13 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys69-Lys967) of human ANPEP/CD13 (Accession #NP_001141.2) fused with an 8×His tag at the C-terminus. Bio-Activity Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >4300 pmol/min/μg. Purity > 98% by SDS-PAGE. Endotoxin < 0.1 EU/μg of the protein by LAL method. Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Species Human Background
Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma.Product information
Synonym APN; CD13; GP150; LAP1; P150; PEPN Expression Host HEK293 cells Tag C-His Swiss-Prot Gene ID Usage guide
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. * For research use only. Not for therapeutic or diagnostic purposes.
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