быстрая доставка ~4 недели
Размер
- 10 μg
- 100 μg
- 20 μg
- 50 μg
- 500 μg
Recombinant Human CDH1/E-Cadherin/CD324 Protein (RP01421)
- Описание
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Overview
Product name Recombinant Human CDH1/E-Cadherin/CD324 Protein Catalog No. RP01421 Description Recombinant Human CDH1/E-Cadherin/CD324 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp155-Ala709) of human E-Cadherin/Cadherin-1 (Accession #NP_004351.1) fused with a 6×His tag at the C-terminus. Bio-Activity Measured by its binding ability in a functional ELISA. Immobilized Human CDH1 at 1 μg/mL (100 μL/well) can bind Human CTNNB1 with a linear range of 0.1-0.78 μg/mL. Purity > 95% by SDS-PAGE. Endotoxin < 0.1 EU/μg of the protein by LAL method. Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Species Human Background
Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.Product information
Synonym Cadherin-1; CDH1; CD324; E-cadherin; Arc-1; ECAD; LCAM; UVO; E-CAD; Cadherin 1; CDH-1 Expression Host HEK293 cells Tag C-His Swiss-Prot Gene ID Usage guide
Storage Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. * For research use only. Not for therapeutic or diagnostic purposes.
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