быстрая доставка ~4 недели
Размер
- 10 μg
- 1000 μg
- 50 μg
- 500 μg
Recombinant Human Visfatin/Nampt Protein (RP00724)
- Описание
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Overview
Product name Recombinant Human Visfatin/Nampt Protein Catalog No. RP00724 Description Recombinant Human Visfatin/Nampt Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-His491) of human Pre-B-Cell Colony-Enhancing Factor 1/PBEF/Visfatin (Accession #P43490) fused with a 6×His tag at the N-terminus. Purity > 95% by SDS-PAGE. Endotoxin < 1 EU/μg of the protein by LAL method. Formulation Lyophilized from a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, pH8.0.Contact us for customized product form or formulation. Species Human Background
Pre-B cell colony enhancing factor (PBEF) was originally identified as a cytokine that potentiated the clonalexpansion and differentiation of pre-B cells, but it is also acknowledged to be the ubiquitous intracellularenzyme nicotinamide phosphoribosyltranferase (NAMPT) and the adipokine “ visfatin ” . PBEF is constitutivelyexpressed in the fetal membranes where its greatest expression is in the amnion. It has intracellular andextracellular forms. Most of the intracellular functions of PBEF are due to its role as a Nampt which can induceangiogenesis through upregulation of VEGF and VEGFR and secretion of MCP-1. Extracellular PBEF has beenshown to increase inflammatory cytokines, such as TNF- α , IL-1 β , IL-16, and TGF- β 1. PBEF also increases theproduction of IL-6, TNF- α , and IL-1 β in CD14+ monocyctes, macrophages, and dendritic cells, enhances theeffectiveness of T cells.Product information
Synonym Pre-B cell-enhancing factor; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Visfatin; NAMPT; PBEF; PBEF1 Expression Host E. coli Tag N-His Swiss-Prot Gene ID Usage guide
Storage Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. * For research use only. Not for therapeutic or diagnostic purposes.
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