![[KO Validated] βIII-Tubulin Rabbit pAb (A17074)](/upload/resize_cache/iblock/664/340_340_140cd750bba9870f18aada2478b24840a/au8eew8gmyorhxztwhklmf8ujj7ihw52.jpg "[KO Validated] βIII-Tubulin Rabbit pAb (A17074)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] βIII-Tubulin Rabbit pAb (A17074)
- Описание
- Характеристики
-
Overview
Product name [KO Validated] βIII-Tubulin Rabbit pAb Catalog No. A17074 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.Immunogen information
Immunogen Recombinant protein of human βIII-Tubulin/β3-Tubulin. Sequence Email for sequence Gene ID Swiss Prot Synonyms CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A Calculated MW 50kDa Observed MW 55kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa, 293T, MCF7, mouse brain, mouse testis, rat brain, rat testis Cellular location Cytoplasm, cytoskeleton Customer validation IF(Homo sapiens)
Western blot analysis of extracts of various cell lines, using βIII-Tubulin/β3-Tubulin antibody (A17074) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts from normal (control) and βIII-Tubulin/β3-Tubulin Rabbit pAb knockout (KO) HeLa cells, using βIII-Tubulin/β3-Tubulin Rabbit pAb antibody (A17074) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of Mouse brain using TUBB3 antibody (A17074) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Rat brain using TUBB3 antibody (A17074) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
-
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human βIII-Tubulin/β3-Tubulin. KO Validated Validated Isotype IgG