![[KO Validated] PIP5K1A Rabbit pAb (A18025)](/upload/resize_cache/iblock/761/340_340_140cd750bba9870f18aada2478b24840a/4e85a6tbejenwkgjitpm4su165036pxa.jpg "[KO Validated] PIP5K1A Rabbit pAb (A18025)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] PIP5K1A Rabbit pAb (A18025)
- Описание
- Характеристики
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Overview
Product name [KO Validated] PIP5K1A Rabbit pAb Catalog No. A18025 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Enables 1-phosphatidylinositol-4-phosphate 5-kinase activity and kinase binding activity. Involved in several processes, including activation of GTPase activity; focal adhesion assembly; and ruffle assembly. Located in several cellular components, including lamellipodium; nuclear speck; and ruffle membrane. Colocalizes with mRNA cleavage and polyadenylation specificity factor complex.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 231-500 of human PIP5K1A (NP_001129109.1). Sequence MNNLLPRSVKMHIKYDLKGSTYKRRASQKEREKPLPTFKDLDFLQDIPDGLFLDADMYNALCKTLQRDCLVLQSFKIMDYSLLMSIHNIDHAQREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDHMGGIPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPGFYAERFQRFMCNTVFKKIPCVHLGRPDVLPQTPPLEEISEGSPIPDPSFSPLVGETLQMLTTSTTLEKLEVAESEFTH Gene ID Swiss Prot Synonyms PIP5K1A Calculated MW 63kDa Observed MW 63kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T Cellular location Cell membrane, Cell projection, Cytoplasm, Nucleus speckle, ruffle 
Western blot analysis of extracts from normal (control) and PIP5K1A knockout (KO) 293T cells, using PIP5K1A antibody (A18025) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 231-500 of human PIP5K1A (NP_001129109.1). KO Validated Validated Isotype IgG