![[KO Validated] TRIM21/SS-A Rabbit pAb (A18027)](/upload/resize_cache/iblock/a18/340_340_140cd750bba9870f18aada2478b24840a/4sh9xwcjbtuaar5oyzzv68j2rs2u37zg.jpg "[KO Validated] TRIM21/SS-A Rabbit pAb (A18027)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] TRIM21/SS-A Rabbit pAb (A18027)
- Описание
- Характеристики
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Overview
Product name [KO Validated] TRIM21/SS-A Rabbit pAb Catalog No. A18027 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 80-330 of human TRIM21/SS-A (NP_003132.2). Sequence SQEAREGTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPM Gene ID Swiss Prot Synonyms SSA; RO52; SSA1; RNF81; Ro/SSA Calculated MW 54kDa Observed MW 50kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T Cellular location Cytoplasm, Cytoplasmic vesicle, Nucleus, P-body, autophagosome 
Western blot analysis of extracts from normal (control) and TRIM21/SS-A knockout (KO) A-549 cells, using TRIM21/SS-A antibody (A18027) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of 293T, using TRIM21/SS-A Rabbit pAb (A18027) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 80-330 of human TRIM21/SS-A (NP_003132.2). KO Validated Validated Isotype IgG