![[KO Validated] PSIP1 Rabbit pAb (A18037)](/upload/resize_cache/iblock/a8d/340_340_140cd750bba9870f18aada2478b24840a/n3lhuh7efx9156m1jeye2lkw399tuaa5.jpg "[KO Validated] PSIP1 Rabbit pAb (A18037)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] PSIP1 Rabbit pAb (A18037)
- Описание
- Характеристики
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Overview
Product name [KO Validated] PSIP1 Rabbit pAb Catalog No. A18037 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Enables DNA-binding transcription factor binding activity; chromatin binding activity; and transcription coactivator activity. Involved in mRNA 5'-splice site recognition and positive regulation of transcription by RNA polymerase II. Located in heterochromatin; nuclear periphery; and nucleoplasm.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 301-530 of human PSIP1 (NP_150091.2). Sequence NMLKGQHEKEAADRKRKQEEQMETEQQNKDEGKKPEVKKVEKKRETSMDSRLQRIHAEIKNSLKIDNLDVNRCIEALDELASLQVTMQQAQKHTEMITTLKKIRRFKVSQVIMEKSTMLYNKFKNMFLVGEGDSVITQVLNKSLAEQRQHEEANKTKDQGKKGPNKKLEKEQTGSKTLNGGSDAQDGNQPQHNGESNEDSKDNHEASTKKKPSSEERETEISLKDSTLDN Gene ID Swiss Prot Synonyms p52; p75; PAIP; DFS70; LEDGF; PSIP2 Calculated MW 60kDa Observed MW 75kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location Nucleus 
Western blot analysis of extracts from normal (control) and PSIP1 knockout (KO) 293T cells, using PSIP1 antibody (A18037) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 301-530 of human PSIP1 (NP_150091.2). KO Validated Validated Isotype IgG