![[KO Validated] XRCC4 Rabbit pAb (A18046)](/upload/resize_cache/iblock/531/340_340_140cd750bba9870f18aada2478b24840a/46kqq5uxemtq5gvzhlr5w9z20ae1o7p5.jpg "[KO Validated] XRCC4 Rabbit pAb (A18046)")
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Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] XRCC4 Rabbit pAb (A18046)
- Описание
- Характеристики
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Overview
Product name [KO Validated] XRCC4 Rabbit pAb Catalog No. A18046 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternate transcript variants such as NM_022406 are unlikely to be expressed in some individuals due to a polymorphism (rs1805377) in the last splice acceptor site.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human XRCC4 (NP_071801.1). Sequence MERKISRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKENSRPDSSLPETSKKEHISAENMSLETLRNSSPEDLFDEI Gene ID Swiss Prot Synonyms SSMED; hXRCC4 Calculated MW 38kDa Observed MW 45kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:10 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa Cellular location Nucleus Customer validation IF(Homo sapiens)
Western blot analysis of extracts from normal (control) and XRCC4 knockout (KO) HeLa cells, using XRCC4 antibody (A18046) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human XRCC4 (NP_071801.1). KO Validated Validated Isotype IgG