![[KO Validated] PRMT8 Rabbit pAb (A18440)](/upload/resize_cache/iblock/935/340_340_140cd750bba9870f18aada2478b24840a/900j3rkhua14tz82kyo0xl2jh50tuw0t.jpg "[KO Validated] PRMT8 Rabbit pAb (A18440)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] PRMT8 Rabbit pAb (A18440)
- Описание
- Характеристики
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Overview
Product name [KO Validated] PRMT8 Rabbit pAb Catalog No. A18440 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 200-380 of human PRMT8 (NP_062828.3). Sequence VIFARDKWLKPGGLMFPDRAALYVVAIEDRQYKDFKIHWWENVYGFDMTCIRDVAMKEPLVDIVDPKQVVTNACLIKEVDIYTVKTEELSFTSAFCLQIQRNDYVHALVTYFNIEFTKCHKKMGFSTAPDAPYTHWKQTVFYLEDYLTVRRGEEIYGTISMKPNAKNVRDLDFTVDLDFKG Gene ID Swiss Prot Synonyms HRMT1L3; HRMT1L4 Calculated MW 45kDa Observed MW 42kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, Mouse brain, Rat brain Cellular location Cell membrane, Cytoplasmic side, Lipid-anchor 
Western blot analysis of extracts from normal (control) and PRMT8 Rabbit pAb knockout (KO) 293T cells, using PRMT8 Rabbit pAb antibody (A18440) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 200-380 of human PRMT8 (NP_062828.3). KO Validated Validated Isotype IgG