![[KO Validated] PNP Rabbit pAb (A19885)](/upload/resize_cache/iblock/716/340_340_140cd750bba9870f18aada2478b24840a/wr6au4kjlsqrf5odl4euc36m8u1qmqj8.jpg "[KO Validated] PNP Rabbit pAb (A19885)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] PNP Rabbit pAb (A19885)
- Описание
- Характеристики
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Overview
Product name [KO Validated] PNP Rabbit pAb Catalog No. A19885 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human PNP (NP_000261.2). Sequence MENGYTYEDYKNTAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYGEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILMASIPLPDKAS Gene ID Swiss Prot Synonyms NP; PUNP; PRO1837 Calculated MW 32kDa Observed MW 30kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location Cytoplasm, cytoskeleton 
Western blot analysis of extracts from normal (control) and PNP knockout (KO) 293T cells, using PNP antibody (A19885) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human PNP (NP_000261.2). KO Validated Validated Isotype IgG