![[KO Validated] TWF2 Rabbit pAb (A19897)](/upload/resize_cache/iblock/576/340_340_140cd750bba9870f18aada2478b24840a/vbmgcrb290qm74j6lfvhc8075z6a8fsq.jpg "[KO Validated] TWF2 Rabbit pAb (A19897)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] TWF2 Rabbit pAb (A19897)
- Описание
- Характеристики
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Overview
Product name [KO Validated] TWF2 Rabbit pAb Catalog No. A19897 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human TWF2 (NP_009215.1). Sequence MAHQTGIHATEELKEFFAKARAGSVRLIKVVIEDEQLVLGASQEPVGRWDQDYDRAVLPLLDAQQPCYLLYRLDSQNAQGFEWLFLAWSPDNSPVRLKMLYAATRATVKKEFGGGHIKDELFGTVKDDLSFAGYQKHLSSCAAPAPLTSAERELQQIRINEVKTEISVESKHQTLQGLAFPLQPEAQRALQQLKQKMVNYIQMKLDLERETIELVHTEPTDVAQLPSRVPRDAARYHFFLYKHTHEGDPLESVVFIYSMPGYKCSIKERMLYSSCKSRLLDSVEQDFHLEIAKKIEIGDGAELTAEFLYDEVHPKQHAFKQAFAKPKGPGGKRGHKRLIRGPGENGDDS Gene ID Swiss Prot Synonyms A6r; A6RP; PTK9L; MSTP011 Calculated MW 40kDa Observed MW 40kDa Applications
Reactivity Human, Mouse Tested applications WB IP Recommended dilution - WB 1:500 - 1:2000
- IP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples 293T Cellular location Cell projection, Cytoplasm, cytoskeleton, perinuclear region, stereocilium 
Western blot analysis of extracts from normal (control) and TWF2 knockout (KO) 293T cells, using TWF2 antibody (A19897) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human TWF2 (NP_009215.1). KO Validated Validated Isotype IgG