![[KO Validated] PYGB Rabbit pAb (A19910)](/upload/resize_cache/iblock/c64/340_340_140cd750bba9870f18aada2478b24840a/3mcalqx05l2cvlvjf8psrqmoqio7ssyg.jpg "[KO Validated] PYGB Rabbit pAb (A19910)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] PYGB Rabbit pAb (A19910)
- Описание
- Характеристики
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Overview
Product name [KO Validated] PYGB Rabbit pAb Catalog No. A19910 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 544-843 of human PYGB (NP_002853.2). Sequence LKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLVTSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFKDIVNMLMHHDRFKVFADYEAYMQCQAQVDQLYRNPKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPPNIPRD Gene ID Swiss Prot Synonyms GPBB Calculated MW 97kDa Observed MW 97kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IP Recommended dilution - WB 1:500 - 1:2000
- IP 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples HeLa Cellular location cytoplasm, extracellular exosome, extracellular region 
Western blot analysis of extracts from normal (control) and PYGB knockout (KO) HeLa cells, using PYGB antibody (A19910) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 544-843 of human PYGB (NP_002853.2). KO Validated Validated Isotype IgG